This one is from S1 E12 “Mattress”
It’s cruel, it’s over-the-top and it’s so, so wrong (everything that Sue normally is), but she says it and says it good!
You know, Q, I’ve forgotten just how ruthless you really are. You’re like a young Sue Sylvester. Now get out of my office, if you can manage to squeeze through the door without your water breaking all over my new carpet.